MAP2K5 Monoclonal Antibody

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Home > Product > Antibody > MAP2K5 Monoclonal Antibody

MAP2K5 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA4461

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human MAP2K5 (AA: 63-180) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

Uniprot accession

Q13163:;

Molecular weight

50kDa

Gene ID

5607

Synonyms

MEK5;MAPKK5;PRKMK5;HsT17454

Research area

Cancer,Cardiovascular,Signal transduction

Target protein

MAP2K5

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using MAP2K5 mAb against human MAP2K5 (AA: 63-180) recombinant protein. (Expected MW is 39 kDa)

Western blot analysis using MAP2K5 mAb against HEK293 (1) and MAP2K5 (AA: 63-180)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using MAP2K5 mouse mAb against Jurkat (1), A431 (2), A549 (3) cell lysate.

Flow cytometric analysis of Jurkat cells using MAP2K5 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MAP2K5 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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