PPP1CB Monoclonal Antibody

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Blood coagulation
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Home > Product > Antibody > PPP1CB Monoclonal Antibody

PPP1CB Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4489

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human PPP1CB (AA: 174-327) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed

Uniprot accession

P62140:;

Molecular weight

37.2kDa

Gene ID

5500

Synonyms

PP1B;PP-1B;PPP1CD;PP1beta

Research area

Signal transduction,Metabolism,Cancer

Target protein

PPP1CB

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using PPP1CB mAb against human PPP1CB (AA: 174-327) recombinant protein. (Expected MW is 43.2 kDa)

Western blot analysis using PPP1CB mAb against HEK293 (1) and PPP1CB (AA: 174-327)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using PPP1CB mouse mAb against Jurkat (1), A431 (2), Hela (3), HepG2 (4), HEK293 (5), MCF-7 (6) cell lysate.

Immunofluorescence analysis of MCF-7 cells using PPP1CB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Jurkat cells using PPP1CB mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PPP1CB mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PPP1CB mouse mAb with DAB staining.

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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