4E-BP1 Polyclonal Antibody

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Home > Product > Antibody > 4E-BP1 Polyclonal Antibody

4E-BP1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Mouse, Rat, Monkey

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse, Rat, Monkey

Applications: WB, IHC-p, ELISA

Product overview

Full product name

4E-BP1 Antibody

Code

BT-AP00038

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from mouse 4E-BP1. AA range:35-84

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

EIF4EBP1 encodes one member (eukaryotic translation initiation factor 4E binding protein 1) of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Uniprot accession

Human:;Rat:;

Molecular weight

12449

Gene ID

Mouse: 13685; Rat: 116636

Synonyms

EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

Research area

Others

Target protein

EIF4EBP1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 5000

Images

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using 4E-BP1 Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from COS7 cells, treated with EGF 200ng/ml 30', using 4E-BP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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