AF-10 Polyclonal Antibody

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Home > Product > Antibody > AF-10 Polyclonal Antibody

AF-10 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

AF-10 Antibody

Code

BT-AP00282

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human AF10. AA range:211-260

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

MLLT10 encodes a transcription factor (myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10) and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for MLLT10.

Uniprot accession

Human:;Mouse:;

Molecular weight

109026

Gene ID

Human: 8028; Mouse: 17354

Synonyms

MLLT10; AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein

Research area

Epigenetics and Nuclear Signaling

Target protein

MLLT10

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 10000

Images

Western Blot analysis of various cells using AF-10 Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from COLO205 and Jurkat cells, using AF10 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of Hela cell. 1,AF-10 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). COX IV Monoclonal Antibody(6C8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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