AFP Polyclonal Antibody

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Home > Product > Antibody > AFP Polyclonal Antibody

AFP Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, ELISA

Product overview

Full product name

AFP Antibody

Code

BT-AP00288

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human AFP. AA range:371-420

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

AFP encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. Alpha-fetoprotein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Uniprot accession

Human:;Mouse:;

Molecular weight

68678

Gene ID

Human: 174

Synonyms

AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

Research area

Cardiovascular; Cancer

Target protein

AFP

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 300
ELISA	1: 20000

Images

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100

Western Blot analysis of mouse liver, mouse serum cells using AFP Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100

Western blot analysis of lysate from mouse liver cells, using AFP Antibody.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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