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Home > Product > Antibody > AKAP 149 Polyclonal Antibody

AKAP 149 Polyclonal Antibody

AKAP 149 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

AKAP 149 Antibody

Code

BT-AP00322

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human AKAP1. AA range:281-330

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. AKAP1 encodes a member of the AKAP family. The encoded protein (A-kinase anchoring protein 1) binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

Uniprot accession

Molecular weight

97342

Gene ID

Human: 8165

Synonyms

AKAP1; AKAP149; PRKA1; A-kinase anchor protein 1; mitochondrial; A-kinase anchor protein 149 kDa; AKAP 149; Dual specificity A-kinase-anchoring protein 1; D-AKAP-1; Protein kinase A-anchoring protein

Research area

Signal Transduction

Target protein

AKAP1

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 5000

Images

Western blot analysis of lysates from HUVEC and COLO cells, using AKAP1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using AKAP 149 Polyclonal Antibody diluted at 1:2000
Immunofluorescence analysis of COS7 cells, using AKAP1 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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