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Home > Product > Antibody > ATP-citrate synthase Polyclonal Antibody

ATP-citrate synthase Polyclonal Antibody

ATP-citrate synthase Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IF, ELISA

Product overview

Full product name

ATP-citrate synthase Antibody

Code

BT-AP00751

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human ATP-Citrate Lyase. AA range:420-469

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for ACLY.

Uniprot accession

Molecular weight

120839

Gene ID

Human: 47; Mouse: 104112; Rat: 24159

Synonyms

ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme

Research area

Signal Transduction; Metabolism

Target protein

ACLY

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IF 1: 200 - 1: 1000
ELISA 1: 10000

Images

Western blot analysis of lysates from COS7 cells, treated with Calyculin 50nM 30', using ATP-Citrate Lyase Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of COS7 cells using ATP-citrate synthase Polyclonal Antibody diluted at 1:1000
Western Blot analysis of various cells using ATP-citrate synthase Polyclonal Antibody diluted at 1:1000

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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