Bcl-2 Polyclonal Antibody

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Home > Product > Antibody > Bcl-2 Polyclonal Antibody

Bcl-2 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Bcl-2 Antibody

Code

BT-AP00858

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human BCL-2. AA range:46-95

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

BCL2 (BCL2, apoptosis regulator) encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

26266

Gene ID

Human: 596; Mouse: 12043

Synonyms

BCL2; Apoptosis regulator Bcl-2

Research area

Cell Biology

Target protein

BCL2

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 10000

Images

Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour).

Western Blot analysis of 3T3 cells using Bcl-2 Polyclonal Antibody diluted at 1:1000

Immunofluorescence analysis of HepG2 cells, using BCL-2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Bcl-2 Polyclonal Antibody diluted at 1:1000

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using BCL-2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, using BCL-2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 1)mouse-liver, 2)mouse-brain, 3)mouse-lung, 4)mcf-7 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (Immunoway:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37° 1hour.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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