Crk II Polyclonal Antibody

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Home > Product > Antibody > Crk II Polyclonal Antibody

Crk II Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Crk II Antibody

Code

BT-AP02234

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CrkII. AA range:187-236

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

CRK encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

33872

Gene ID

Human: 1398; Mouse: 12928; Rat: 54245

Synonyms

CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38

Research area

Signal Transduction

Target protein

CRK

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CrkII Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COS7 cells, using CrkII Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HUVEC cells, using CrkII Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Crk II Polyclonal Antibody

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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