FN1 Polyclonal Antibody

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Home > Product > Antibody > FN1 Polyclonal Antibody

FN1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

FN1 Antibody

Code

BT-AP03292

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Fibronectin 1. AA range:2337-2386

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

FN1 encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

262607

Gene ID

Human: 2335; Mouse: 14268

Synonyms

FN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG

Research area

Cardiovascular

Target protein

FN1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 20000

Images

Western blot analysis of lysates from 1) 3T3 , 2) KB ,3) K562 ,4) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using Fibronectin 1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of HeLa cells using FN1 Polyclonal Antibody diluted at 1:2000

Western blot analysis of lysates from HeLa cells, using Fibronectin 1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using FN1 Polyclonal Antibody diluted at 1:2000

Western blot analysis of 3T3 KB K562 293T lysis using FN1 antibody. Antibody was diluted at 1:2000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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