FoxO3A Polyclonal Antibody

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Home > Product > Antibody > FoxO3A Polyclonal Antibody

FoxO3A Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

FoxO3A Antibody

Code

BT-AP03334

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human FKHRL1. AA range:220-269

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

FOXO3 belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. FOXO3 likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Uniprot accession

Human:;Mouse:;

Molecular weight

71277

Gene ID

Human: 2309; Mouse: 56484

Synonyms

FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1

Research area

Epigenetics and Nuclear Signaling

Target protein

FOXO3

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FKHRL1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of Jurkat cells using FoxO3A Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from COLO205 cells, treated with serum, using FKHRL1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 1)MCF-7 , 2)COLO205, 3) HELA cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.

Western Blot analysis of various cells using FoxO3A Polyclonal Antibody diluted at 1:1000

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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