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Home > Product > Antibody > Histone H4 (Acetyl Lys12) Polyclonal Antibody

Histone H4 (Acetyl Lys12) Polyclonal Antibody

Histone H4 (Acetyl Lys12) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Histone H4 (Acetyl Lys12) Antibody

Code

BT-AP04100

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12. AA range:10-59

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST4H4 is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.

Uniprot accession

Molecular weight

11367

Gene ID

Human: 121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370; Mouse: 1.00041e+008; Rat: 1.00361e+008

Synonyms

HIST1H4A; H4/A; H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G; H4FG; HIST1H4D; H4/B; H4FB; HIST1H4E; H4/J; H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E; H4FE; HI

Research area

Epigenetics and Nuclear Signaling

Target protein

HIST1H4A

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 10000

Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot analysis of KB cells using Acetyl-Histone H4 (K12) Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
Western blot analysis of Mouse-kidney HELA lysis using Acetyl-Histone H4 (K12) antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
Immunofluorescence analysis of Hela cell. 1,Histone H4 (Acetyl Lys12) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
Western Blot analysis of 293 cells using Acetyl-Histone H4 (K12) Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys12) Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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