M-CSF Polyclonal Antibody

Product overview

Full product name

M-CSF Antibody

Code

BT-AP05287

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from the C-terminal region of human CSF1. AA range:505-554

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Macrophage colony-stimulating factor 1 encoded by CSF1 is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants.

Uniprot accession

Human:;Mouse:;

Molecular weight

60119

Gene ID

Human: 1435

Synonyms

CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim

Research area

Immunology

Target protein

CSF1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 10000

Images

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western Blot analysis of 3T3 cells using M-CSF Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Western Blot analysis of NIH-3T3, Hela cells using M-CSF Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

Customer reviews and Q&As

No reviews

Q&A

No reviews

Q&A