Rpb1 Polyclonal Antibody

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Home > Product > Antibody > Rpb1 Polyclonal Antibody

Rpb1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Rpb1 Antibody

Code

BT-AP07994

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human POLR2A. AA range:1585-1634

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

POLR2A encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Uniprot accession

Human:;Mouse:;

Molecular weight

217206

Gene ID

Human: 5430; Mouse: 20020

Synonyms

POLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA po

Research area

Epigenetics and Nuclear Signaling

Target protein

POLR2A

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 10000

Images

Western Blot analysis of various cells using Rpb1 Polyclonal Antibody diluted at 1:1000 cells nucleus.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using POLR2A Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using POLR2A Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COS7 cells, treated with EGF 200ng/ml 30', using POLR2A Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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