TdT Polyclonal Antibody

Product overview

Full product name

TdT Antibody

Code

BT-AP08911

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human DNTT. AA range:381-430

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

DNTT is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Uniprot accession

Human:;Mouse:;

Molecular weight

58437

Gene ID

Human: 1791

Synonyms

DNTT; TDT; DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase

Research area

Immunology

Target protein

DNTT

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
ELISA	1: 40000
IHC-p	100 - 300

Images

Immunohistochemical analysis of paraffin-embedded Human thymus. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Thyroid. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western blot analysis of DNTT Antibody. The lane on the right is blocked with the DNTT peptide.

Western Blot analysis of HepG2 cells using TdT Polyclonal Antibody cells nucleus.

Immunohistochemical analysis of paraffin-embedded Human Thyroid. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human thymus. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

Customer reviews and Q&As

No reviews

Q&A

No reviews

Q&A