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Home > Product > ELISA Primacu > Human Cd44 Antigen, CD44 Primacu™ ELISA Kit

Human Cd44 Antigen, CD44 Primacu™ ELISA Kit

Human Cd44 Antigen, CD44 Primacu™ ELISA Kit

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Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 36.66 pg/mL

Detection range: 125-8000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 36.66 pg/mL

Detection range: 125-8000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

CD44 antigen

Code

BPE248

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

36.66 pg/mL

Detection range

125-8000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.

UniProt accession

MASS(Da)

81538

GeneID

960

Synonyms

LHR;MDU2;MDU3;MIC4

Gene names

CD44

Research area

Immunology,Cancer,Stem cells

Target protein

CD44

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE248 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8000 pg/ml 3.207 3.357 3.249
3.508
4000 pg/ml 1.814 1.815 1.707
1.817
2000 pg/ml 0.789 0.816 0.708
0.844
1000 pg/ml 0.402 0.394 0.286
0.387
500 pg/ml 0.21 0.218 0.11
0.227
250 pg/ml 0.164 0.158 0.05
0.153
125 pg/ml 0.131 0.128 0.02
0.126
0 pg/ml 0.112 0.108 0
0.105

Precision

We measured random samples of BPE248 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 680 24.4 3.6
Intra-assay 2 20 1619 50.1 3.1
Intra-assay 3 20 5584 294 5.3
Inter-assay 1 20 681 56.9 8.3
Inter-assay 2 20 1627 102 6.3
Inter-assay 3 20 5529 392 7.1

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE248

    $2040.00

  • 96T

    BPE248

    $480.00

  • 15*96T

    BPE248

    $5400.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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