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Home > Product > ELISA Primacu > Mouse Tumor Necrosis Factor, Tnf Primacu™ ELISA Kit

Mouse Tumor Necrosis Factor, Tnf Primacu™ ELISA Kit

Mouse Tumor Necrosis Factor, Tnf Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 43 pg/mL

Detection range: 93.75-6000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 43 pg/mL

Detection range: 93.75-6000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Tumor necrosis factor

Code

BPE310

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

43 pg/mL

Detection range

93.75-6000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & Antibody Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

MASS(Da)

25896

GeneID

21926

Synonyms

Tnfa;Tnfsf2

Gene names

Tnf

Research area

Cancer,Cardiovascular,Immunology,Metabolism,Microbiology,Neuroscience,Signal transduction

Target protein

TNF-alpha

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE310 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6000 pg/ml 2.407 2.523 2.392
2.639
3000 pg/ml 1.466 1.474 1.343
1.482
1500 pg/ml 0.865 0.919 0.788
0.973
750 pg/ml 0.546 0.553 0.422
0.56
375 pg/ml 0.341 0.357 0.226
0.373
187.5 pg/ml 0.249 0.248 0.117
0.248
93.75 pg/ml 0.189 0.186 0.055
0.183
0 pg/ml 0.138 0.131 0
0.124

Precision

We measured random samples of BPE310 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 252 16.6 6.6
Intra-assay 2 20 811 37.8 4.7
Intra-assay 3 20 3528 107 3
Inter-assay 1 20 254 18.4 7.3
Inter-assay 2 20 814 42.2 5.2
Inter-assay 3 20 3544 159 4.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE310

    $2040.00

  • 96T

    BPE310

    $480.00

  • 15*96T

    BPE310

    $5400.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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