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Home > Product > ELISA Primacu > Human 15-hydroxyprostaglandin dehydrogenase [NAD(+)], HPGD Primacu™ ELISA Kit

Human 15-hydroxyprostaglandin dehydrogenase [NAD(+)], HPGD Primacu™ ELISA Kit

Human 15-hydroxyprostaglandin dehydrogenase [NAD(+)], HPGD Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.44 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.44 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

15-hydroxyprostaglandin dehydrogenase [NAD(+)]

Code

BPE185

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

6.44 pg/mL

Detection range

31.25-2000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone, and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoarthropathy autosomal recessive (PHOAR), cranio-osteoarthropathy (COA), and isolated congenital nail clubbing.

UniProt accession

MASS(Da)

28977

GeneID

3248

Synonyms

PGDH1;SDR36C1

Gene names

HPGD

Research area

Immunology,Signal transduction,Neuroscience

Target protein

15-PGDH

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE185 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000 pg/ml 2.431 2.322 2.241
2.214
1000 pg/ml 1.351 1.276 1.195
1.201
500 pg/ml 0.634 0.647 0.566
0.66
250 pg/ml 0.372 0.357 0.276
0.343
125 pg/ml 0.225 0.213 0.132
0.202
62.5 pg/ml 0.139 0.134 0.053
0.129
31.25 pg/ml 0.113 0.108 0.027
0.103
0 pg/ml 0.077 0.081 0
0.085

Precision

We measured random samples of BPE185 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 153 9.1 6
Intra-assay 2 20 413 16.6 4
Intra-assay 3 20 1115 86 7.7
Inter-assay 1 20 154 12.9 8.4
Inter-assay 2 20 417 35.7 8.6
Inter-assay 3 20 1127 110 9.8

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE185

    $2320.00

  • 96T

    BPE185

    $580.00

  • 15*96T

    BPE185

    $6525.00

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Lead time: Within two weeks

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