Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE348 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1500 pg/ml	3.052	2.911	2.779
	2.77
750 pg/ml	1.69	1.684	1.552
	1.678
375 pg/ml	0.914	0.935	0.803
	0.957
187.5 pg/ml	0.588	0.556	0.424
	0.524
93.75 pg/ml	0.338	0.36	0.228
	0.382
46.875 pg/ml	0.259	0.248	0.116
	0.238
23.4375 pg/ml	0.183	0.19	0.058
	0.197
0 pg/ml	0.125	0.132	0
	0.139

We measured random samples of BPE348 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	188	19.2	10.2
Intra-assay	2	20	460	16.5	3.6
Intra-assay	3	20	1179	40.9	3.5
Inter-assay	1	20	187	17.6	9.4
Inter-assay	2	20	464	36.1	7.8
Inter-assay	3	20	1177	94.8	8.1

> Protocol Booklet

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