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Home > Product > ELISA Primacu > Human Vascular Endothelial Growth Factor A, VEGF Primacu™ ELISA Kit

Human Vascular Endothelial Growth Factor A, VEGF Primacu™ ELISA Kit

Human Vascular Endothelial Growth Factor A, VEGF Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.56 pg/mL

Detection range: 18.75-1200 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.56 pg/mL

Detection range: 18.75-1200 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Vascular endothelial growth factor A

Code

BPE172

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

8.56 pg/mL

Detection range

18.75-1200 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

MASS(Da)

27042

GeneID

7422

Synonyms

VEGF

Gene names

VEGFA

Research area

Cancer,Cardiovascular,Metabolism,Neuroscience,Signal transduction,Stem cells

Target protein

VEGF165

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE172 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200 pg/ml 2.818 2.709 2.625
2.601
600 pg/ml 1.477 1.442 1.358
1.407
300 pg/ml 0.808 0.802 0.718
0.796
150 pg/ml 0.425 0.445 0.361
0.465
75 pg/ml 0.278 0.264 0.18
0.251
37.5 pg/ml 0.171 0.17 0.086
0.169
18.75 pg/ml 0.121 0.129 0.045
0.138
0 pg/ml 0.087 0.084 0
0.082

Precision

We measured random samples of BPE172 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 91.2 3.2 3.5
Intra-assay 2 20 268 7.9 3
Intra-assay 3 20 654 19.1 2.9
Inter-assay 1 20 92 7.6 8.2
Inter-assay 2 20 270 22 8.2
Inter-assay 3 20 655 31.9 4.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE172

    $2040.00

  • 96T

    BPE172

    $480.00

  • 15*96T

    BPE172

    $5400.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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