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Home > Product > ELISA Primacu > Human Interleukin-1 Receptor-Like 1, IL1RL1 Primacu™ ELISA Kit

Human Interleukin-1 Receptor-Like 1, IL1RL1 Primacu™ ELISA Kit

Human Interleukin-1 Receptor-Like 1, IL1RL1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.87 pg/mL

Detection range: 7.8-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.87 pg/mL

Detection range: 7.8-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-1 receptor-like 1

Code

BPE020

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

1.87 pg/mL

Detection range

7.8-500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

IL-1 receptor–like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interleukin-1 like 1 (IL1RL1) protein can be used to diagnose cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage proinflammatory function. IL33 is a specific ligand of ST2L and induces the production of Th2 cytokines.

UniProt accession

MASS(Da)

63358

GeneID

9173

Synonyms

DER4;ST2;T1

Gene names

IL1RL1

Research area

Immunology,Cardiovascular

Target protein

ST2

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE020 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
500 pg/ml 2.415 2.34 2.251
2.265
250 pg/ml 1.434 1.385 1.296
1.336
125 pg/ml 0.769 0.773 0.684
0.777
62.5 pg/ml 0.422 0.427 0.338
0.432
31.25 pg/ml 0.262 0.258 0.169
0.254
15.625 pg/ml 0.186 0.174 0.085
0.162
7.8125 pg/ml 0.13 0.133 0.044
0.136
0 pg/ml 0.084 0.089 0
0.094

Precision

We measured random samples of BPE020 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 52.5 2.7 5.2
Intra-assay 2 20 194 6.1 3.2
Intra-assay 3 20 397 22 5.5
Inter-assay 1 20 52.3 1.8 3.4
Inter-assay 2 20 195 15.8 8.1
Inter-assay 3 20 395 13.4 3.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE020

    $2040.00

  • 96T

    BPE020

    $480.00

  • 15*96T

    BPE020

    $5400.00

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Lead time: Within two weeks

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