Product overview
Full product name
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Detection range
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Background
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.
UniProt accession
MASS（Da）
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Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of BPE397 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 200 pg/ml | 2.526 | 2.593 | 2.511 |
| 2.661 | |||
| 100 pg/ml | 1.402 | 1.366 | 1.284 |
| 1.331 | |||
| 50 pg/ml | 0.686 | 0.728 | 0.646 |
| 0.771 | |||
| 25 pg/ml | 0.42 | 0.404 | 0.322 |
| 0.389 | |||
| 12.5 pg/ml | 0.251 | 0.244 | 0.162 |
| 0.238 | |||
| 6.25 pg/ml | 0.159 | 0.155 | 0.073 |
| 0.152 | |||
| 3.125 pg/ml | 0.112 | 0.116 | 0.034 |
| 0.121 | |||
| 0 pg/ml | 0.079 | 0.082 | 0 |
| 0.086 |
Precision
We measured random samples of BPE397 within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 14.6 | 1.1 | 7.6 |
| Intra-assay | 2 | 20 | 39.4 | 1.7 | 4.4 |
| Intra-assay | 3 | 20 | 158 | 8.8 | 5.6 |
| Inter-assay | 1 | 20 | 14.7 | 1.3 | 8.8 |
| Inter-assay | 2 | 20 | 39.8 | 3.6 | 9 |
| Inter-assay | 3 | 20 | 157 | 13 | 8.3 |
Protocols
References
Customer reviews and Q&As
Associated products
Size
Code
Price
5*96T
BPE397
$2040.00
96T
BPE397
$480.00
15*96T
BPE397
$5400.00
Lead time: Within two weeks
