Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Glutamate Decarboxylase 1, GAD1 ELISA Kit

Rat Glutamate Decarboxylase 1, GAD1 ELISA Kit

Rat Glutamate Decarboxylase 1, GAD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.09ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.09ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Glutamate decarboxylase 1

Code

E1353Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.09ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Glutamate decarboxylase 1 (brain, 67kDa) (GAD67), also known as GAD1, is a human gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

UniProt accession

MASS(Da)

66,640

GeneID

24379

Synonyms

67 kDa glutamic acid decarboxylase;DCE1;gad;GAD1;Gad67;GAD-67;Glutamate decarboxylase 1;Glutamate decarboxylase 67 kDa isoform;SCP ;

Gene names

Gad1

Research area

Signal Transduction; Immunology

Target protein

GAD1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1353Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.228 2.262 2.23
2.297
16ng/ml 1.516 1.516 1.484
1.516
8ng/ml 0.556 0.636 0.604
0.715
4ng/ml 0.432 0.376 0.345
0.321
2ng/ml 0.21 0.199 0.167
0.188
0ng/ml 0.03 0.032 0
0.034

Precision

We measured random samples of E1353Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 21.57 0.79 3.67
Intra-Assay 2 18 12.68 0.54 4.24
Intra-Assay 3 18 7.26 0.28 3.9

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E1353Ra

    $320.00

  • 96T

    E1353Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up