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Home > Product > ELISA Kit > Human Kallistatin, SERPINA4 ELISA Kit

Human Kallistatin, SERPINA4 ELISA Kit

Human Kallistatin, SERPINA4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Kallistatin

Code

E3392Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.

UniProt accession

MASS(Da)

48,542

GeneID

5267

Synonyms

Kallikrein inhibitor;Kallistatin;Peptidase inhibitor 4;PI-4;Serpin A4;SERPINA 4;SERPINA4

Gene names

SERPINA4

Research area

Others

Target protein

SERPINA4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3392Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.32 2.357 2.324
2.393
6ng/ml 1.451 1.323 1.29
1.194
3ng/ml 0.581 0.578 0.546
0.575
1.5ng/ml 0.299 0.316 0.284
0.334
0.75ng/ml 0.161 0.143 0.111
0.125
0ng/ml 0.044 0.032 0
0.021

Precision

We measured random samples of E3392Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.6 0.15 2.2
Intra-Assay 2 18 5.5 0.3 5.5
Intra-Assay 3 18 2.9 0.1 3.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E3392Hu

    $320.00

  • 96T

    E3392Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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