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Home > Product > ELISA Kit > Human Interleukin 24, IL-24 ELISA Kit

Human Interleukin 24, IL-24 ELISA Kit

Human Interleukin 24, IL-24 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.56ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.56ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin 24

Code

E2162Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.56ng/L

Detection range

5-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Interleukin 24 (IL-24) is a protein in the interleukin family, a type of cytokine signaling molecule in the immune system. In humans, this protein is encoded by the IL24 gene. IL-24 is a cytokine belonging to the IL-10 family of cytokines that signals through two heterodimeric receptors: IL-20R1/IL-20R2 and IL-22R1/IL-20R2. This interleukin is also known as melanoma differentiation-associated 7 (mda-7) due to its discovery as a tumour suppressing protein. IL-24 appears to control cell survival and proliferation by inducing rapid activation of particular transcription factors called STAT1 and STAT3. This cytokine is predominantly released by activated monocytes, macrophages and T helper 2 (Th2) cells[5] and acts on skin, lung, and reproductive tissues. IL-24 performs important roles in wound healing, arthritis, psoriasis and cancer. Several studies have shown that cell death occurs in cancer cells/cell lines following exposure to IL-24. The gene for IL-24 is located on chromosome 1 in humans.

UniProt accession

MASS(Da)

23,825

GeneID

11009

Synonyms

C49A;FISP;IL10B;IL-24;Interleukin-24;MDA7;MDA-7;Melanoma differentiation-associated gene 7 protein;MOB5;ST16;Suppression of tumorigenicity 16 protein

Gene names

IL24

Research area

Immunology

Target protein

IL-24

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2162Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.4 2.33 2.271
2.26
400ng/L 1.31 1.308 1.249
1.307
200ng/L 0.881 0.88 0.821
0.878
100ng/L 0.501 0.502 0.443
0.502
50ng/L 0.244 0.275 0.216
0.306
0ng/L 0.046 0.059 0
0.072

Precision

We measured random samples of E2162Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 350.72 16.81 4.79
Intra-Assay 2 18 439.53 10.7 2.43
Intra-Assay 3 18 62.91 3.86 6.14

Protocols

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  • Size

    Code

    Price

  • 48T

    E2162Hu

    $320.00

  • 96T

    E2162Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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