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Home > Product > ELISA Kit > Human Histone Deacetylase 3, HDAC3 ELISA Kit

Human Histone Deacetylase 3, HDAC3 ELISA Kit

Human Histone Deacetylase 3, HDAC3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Histone Deacetylase 3

Code

E3583Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.28ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (PubMed: 21444723, PubMed: 23911289). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed: 25190803). Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner (By similarity). During the activation phase, promotes the accumulation of ubiquitinated ARNTL/BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and ARNTL/BMAL1 (By similarity). The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene ARTNL/BMAL1 and the genes involved in lipid metabolism in the liver (By similarity). Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding (PubMed: 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed: 28167758). Interacts with SETD5 (By similarity).

UniProt accession

MASS(Da)

48,848

GeneID

8841

Synonyms

HD3;HDAC 3;HDAC3;Histone deacetylase 3;RPD3-2;SMAP45

Gene names

HDAC3

Research area

Epigenetics and Nuclear Signaling

Target protein

HDAC3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3583Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 2.232 2.251 2.213
2.27
32ng/ml 1.438 1.426 1.388
1.414
16ng/ml 0.896 0.829 0.79
0.762
8ng/ml 0.409 0.407 0.369
0.406
4ng/ml 0.238 0.265 0.227
0.291
0ng/ml 0.041 0.038 0
0.036

Precision

We measured random samples of E3583Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 33.3 1.42 4.3
Intra-Assay 2 18 15.8 0.72 4.6
Intra-Assay 3 18 11.2 0.5 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E3583Hu

    $320.00

  • 96T

    E3583Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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