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Home > Product > ELISA Kit > Human Serine Threonine-protein Phosphatase PP1-alpha Catalytic Subunit, PPP1CA ELISA Kit

Human Serine Threonine-protein Phosphatase PP1-alpha Catalytic Subunit, PPP1CA ELISA Kit

Human Serine Threonine-protein Phosphatase PP1-alpha Catalytic Subunit, PPP1CA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.95ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.95ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Code

E6153Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.95ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Dephosphorylates CENPA (PubMed:25556658). Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323). (Microbial infection) Necessary for alphaviruses replication.

UniProt accession

MASS(Da)

37,512

GeneID

5499

Synonyms

PP1A;PP-1A;PP1alpha;PPP1A;PPP1CA;Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Gene names

PPP1CA

Research area

Signal Transduction; Cancer; Metabolism

Target protein

PPP1CA

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6153Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.356 2.266 2.213
2.176
800ng/L 1.448 1.498 1.445
1.549
400ng/L 0.848 0.862 0.809
0.877
200ng/L 0.435 0.463 0.41
0.49
100ng/L 0.218 0.252 0.199
0.285
0ng/L 0.051 0.053 0
0.055

Precision

We measured random samples of E6153Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 948.51 43.25 4.56
Intra-Assay 2 18 434.78 22.63 5.2
Intra-Assay 3 18 343.41 17.33 5.05

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E6153Hu

    $320.00

  • 96T

    E6153Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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