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Home > Product > ELISA Kit > Human Coagulation Factor 5, F5 ELISA Kit

Human Coagulation Factor 5, F5 ELISA Kit

Human Coagulation Factor 5, F5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.53ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.53ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Coagulation Factor 5

Code

E4114hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.53ng/mL

Detection range

1-400ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

UniProt accession

MASS(Da)

251,703

GeneID

2153

Synonyms

Activated protein C cofactor;Coagulation factor V;Coagulation factor V heavy chain;Coagulation factor V light chain;F 5;F5;Proaccelerin, labile factor

Gene names

F5

Research area

Blood Coagulation

Target protein

F5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4114hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/mL 2.23 2.165 2.122
2.1
120ng/mL 1.772 1.847 1.805
1.923
60ng/mL 1.132 1.155 1.112
1.178
30ng/mL 0.678 0.673 0.63
0.668
15ng/mL 0.421 0.396 0.353
0.371
0ng/mL 0.047 0.043 0
0.039

Precision

We measured random samples of E4114hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15 0.67 4.5
Intra-Assay 2 18 79.7 3.39 4.2
Intra-Assay 3 18 48.9 2.93 6

Protocols

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  • Size

    Code

    Price

  • 48T

    E4114hu

    $320.00

  • 96T

    E4114hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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