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Home > Product > ELISA Kit > Human Udp-Glucose 4-Epimerase, GALE ELISA Kit

Human Udp-Glucose 4-Epimerase, GALE ELISA Kit

Human Udp-Glucose 4-Epimerase, GALE ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 8.07ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 8.07ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Udp-Glucose 4-Epimerase

Code

E4621Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

8.07ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.

UniProt accession

MASS(Da)

38,282

GeneID

2582

Synonyms

Galactowaldenase;GALE;UDP-galactose 4-epimerase;UDP-GalNAc 4-epimerase;UDP-GlcNAc 4-epimerase;UDP-glucose 4-epimerase;UDP-N-acetylgalactosamine 4-epimerase;UDP-N-acetylglucosamine 4-epimerase

Gene names

GALE

Research area

Others

Target protein

GALE

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4621Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.339 2.33 2.268
2.321
800ng/L 1.72 1.707 1.645
1.693
400ng/L 1.147 1.088 1.026
1.028
200ng/L 0.757 0.703 0.641
0.65
100ng/L 0.486 0.441 0.379
0.397
0ng/L 0.063 0.062 0
0.061

Precision

We measured random samples of E4621Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 640.2 26.92 4.2
Intra-Assay 2 18 143.4 8.21 5.7
Intra-Assay 3 18 345.7 24.2 7

Protocols

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  • Size

    Code

    Price

  • 48T

    E4621Hu

    $320.00

  • 96T

    E4621Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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