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Home > Product > ELISA Kit > Human Histone H2A Type 1-A, HIST1H2AA ELISA Kit

Human Histone H2A Type 1-A, HIST1H2AA ELISA Kit

Human Histone H2A Type 1-A, HIST1H2AA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.71ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.71ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Histone H2A Type 1-A

Code

E5416Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.71ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

UniProt accession

MASS(Da)

14,234

GeneID

221613

Synonyms

H2AC1;H2A-clustered histone 1;Histone H2A type 1-A;Histone H2A/r

Gene names

H2AC1

Research area

Epigenetics and Nuclear Signaling

Target protein

H2AC1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5416Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.184 2.22 2.147
2.256
1600ng/L 1.358 1.362 1.289
1.366
800ng/L 0.737 0.8 0.728
0.864
400ng/L 0.487 0.502 0.43
0.518
200ng/L 0.298 0.306 0.234
0.315
0ng/L 0.071 0.073 0
0.075

Precision

We measured random samples of E5416Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1171.8 64.51 5.5
Intra-Assay 2 18 1690.7 54.75 3.2
Intra-Assay 3 18 710.2 54.66 7.7

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5416Hu

    $320.00

  • 96T

    E5416Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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