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Home > Product > ELISA Kit > Human Histone H4, HIST1H4A ELISA Kit

Human Histone H4, HIST1H4A ELISA Kit

Human Histone H4, HIST1H4A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.33ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.33ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Histone H4

Code

E5420Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.33ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

UniProt accession

MASS(Da)

11,367

GeneID

1.2150455431383E+59

Synonyms

H4C1;Histone H4

Gene names

H4C1

Research area

Epigenetics and Nuclear Signaling

Target protein

H4C1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5420Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.306 2.29 2.245
2.274
1200ng/L 1.205 1.279 1.234
1.354
600ng/L 0.714 0.643 0.597
0.571
300ng/L 0.292 0.337 0.291
0.381
150ng/L 0.205 0.186 0.141
0.168
0ng/L 0.045 0.045 0
0.046

Precision

We measured random samples of E5420Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 341.2 13.78 4
Intra-Assay 2 18 1248.6 30.3 2.4
Intra-Assay 3 18 1117.8 58.25 5.2

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5420Hu

    $320.00

  • 96T

    E5420Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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