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Home > Product > ELISA Kit > Human Sam Domain and Hd Domain-containing Protein 1, SAMHD1 ELISA Kit

Human Sam Domain and Hd Domain-containing Protein 1, SAMHD1 ELISA Kit

Human Sam Domain and Hd Domain-containing Protein 1, SAMHD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.32ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.32ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sam Domain and Hd Domain-Containing Protein 1

Code

E6116Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.32ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Is a positive regulator of NF-kappa-B signaling downstream of TLR4 activation. It acts as a scaffold molecule to assemble a molecular complex that includes TRAF6, MAP3K7, CHUK and IKBKB, thereby facilitating NF-kappa-B signaling activation (PubMed: 23776175). Regulates TRAF6 and MAP3K7 ubiquitination (PubMed: 23776175). Involved in the regulation of cell mobility (PubMed: 23333244, PubMed: 23776175, PubMed: 25315659). Regulates lipolysaccharide (LPS)-induced endothelial cell migration (PubMed: 23776175). Is involved in the regulation of skin pigmentation through the control of melanocyte migration in the epidermis (PubMed: 23333244).

UniProt accession

MASS(Da)

136,653

GeneID

23328

Synonyms

Proline-glutamate repeat-containing protein;SAM and SH3 domain-containing protein 1;SASH 1;SASH1

Gene names

SASH1

Research area

Others

Target protein

SASH1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6116Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.362 2.398 2.336
2.433
800ng/L 1.445 1.386 1.324
1.326
400ng/L 0.758 0.821 0.759
0.884
200ng/L 0.51 0.53 0.468
0.55
100ng/L 0.267 0.294 0.233
0.322
0ng/L 0.063 0.062 0
0.06

Precision

We measured random samples of E6116Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1124 34.2 3
Intra-Assay 2 18 511.3 32.53 6.4
Intra-Assay 3 18 352.7 22.35 6.3

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6116Hu

    $320.00

  • 96T

    E6116Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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