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Home > Product > ELISA Kit > Human Sentrin-specific Protease 1, SENP1 ELISA Kit

Human Sentrin-specific Protease 1, SENP1 ELISA Kit

Human Sentrin-specific Protease 1, SENP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.88ng/L

Detection range: 2-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.88ng/L

Detection range: 2-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sentrin-Specific Protease 1

Code

E6134Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.88ng/L

Detection range

2-700ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Protease that catalyzes two essential functions in the SUMO pathway (PubMed: 10652325, PubMed: 15199155, PubMed: 16253240, PubMed: 16553580, PubMed: 21829689, PubMed: 21965678, PubMed: 23160374, PubMed: 24943844, PubMed: 25406032, PubMed: 29506078). The first is the hydrolysis of an alpha-linked Peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked Peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. Deconjugates SUMO1 from HIPK2 (PubMed: 16253240). Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity (PubMed: 21829689). Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity (PubMed: 23160374). Deconjugates SUMO2 from MTA1 (PubMed: 21965678). Deconjugates SUMO1 from METTL3 (PubMed: 29506078). Desumoylates CCAR2 which decreases its interaction with SIRT1 (PubMed: 25406032). Deconjugates SUMO1 from GPS2 (PubMed: 24943844).

UniProt accession

MASS(Da)

73,481

GeneID

29843

Synonyms

SENP 1;SENP1;Sentrin/SUMO-specific protease SENP1;Sentrin-specific protease 1

Gene names

SENP1

Research area

Cell Biology

Target protein

SENP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6134Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/L 1.961 1.997 1.949
2.033
200ng/L 1.336 1.354 1.306
1.373
100ng/L 0.606 0.576 0.527
0.545
50ng/L 0.311 0.366 0.318
0.422
25ng/L 0.158 0.186 0.138
0.214
0ng/L 0.054 0.048 0
0.043

Precision

We measured random samples of E6134Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 261 10.31 4
Intra-Assay 2 18 112.7 3.84 3.4
Intra-Assay 3 18 83.4 3.45 4.1

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6134Hu

    $320.00

  • 96T

    E6134Hu

    $458.00

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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