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Home > Product > ELISA Kit > Human Slam Family Member 5, CD84 ELISA Kit

Human Slam Family Member 5, CD84 ELISA Kit

Human Slam Family Member 5, CD84 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.048ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.048ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Slam Family Member 5

Code

E6178Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.048ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B (By similarity). Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells (PubMed: 11564780, PubMed: 12115647. PubMed: 12928397, PubMed: 12962726, PubMed: 16037392) Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity (By similarity). In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1 (PubMed: 22068234). In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2 (By similarity). Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8; inhibits TRIM21-mediated proteasomal degradation of IRF8 (PubMed: 29434592).

UniProt accession

MASS(Da)

38,782

GeneID

8832

Synonyms

CD 84;CD antigen CD84;CD84;Cell surface antigen MAX.3;Hly9-beta;Leukocyte differentiation antigen CD84;Signaling lymphocytic activation molecule 5;SLAM family member 5

Gene names

CD84

Research area

Immunology

Target protein

CD84

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6178Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.321 2.339 2.293
2.357
12ng/mL 1.164 1.183 1.137
1.202
6ng/mL 0.768 0.792 0.746
0.816
3ng/mL 0.49 0.462 0.416
0.434
1.5ng/mL 0.228 0.229 0.183
0.23
0ng/mL 0.045 0.046 0
0.047

Precision

We measured random samples of E6178Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.7 0.14 5
Intra-Assay 2 18 8.8 0.51 5.8
Intra-Assay 3 18 14.1 0.47 3.4

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E6178Hu

    $320.00

  • 96T

    E6178Hu

    $458.00

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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