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Home > Product > ELISA Kit > Human Small Ubiquitin-related Modifier 1, SUMO1 ELISA Kit

Human Small Ubiquitin-related Modifier 1, SUMO1 ELISA Kit

Human Small Ubiquitin-related Modifier 1, SUMO1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Small Ubiquitin-Related Modifier 1

Code

E6183Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.93ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium Channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed: 19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed: 24651376).

UniProt accession

MASS(Da)

11,557

GeneID

7341

Synonyms

GAP-modifying protein 1;GMP1;Sentrin;Small ubiquitin-related modifier 1;SMT3 homolog 3;Smt3C;SUMO 1;SUMO1;SUMO-1;Ubiquitin-homology domain protein PIC1;Ubiquitin-like protein SMT3C;Ubiquitin-like protein UBL1

Gene names

SUMO1

Research area

Others

Target protein

SUMO1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6183Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.334 2.361 2.3
2.388
1200ng/L 1.358 1.322 1.261
1.285
600ng/L 0.748 0.725 0.664
0.702
300ng/L 0.419 0.432 0.371
0.445
150ng/L 0.253 0.26 0.199
0.266
0ng/L 0.073 0.061 0
0.049

Precision

We measured random samples of E6183Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 496.8 25.01 5
Intra-Assay 2 18 1607.8 57.9 3.6
Intra-Assay 3 18 732.4 41.4 5.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E6183Hu

    $320.00

  • 96T

    E6183Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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