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Home > Product > ELISA Kit > Human Small Ubiquitin-related Modifier 2, SUMO2 ELISA Kit

Human Small Ubiquitin-related Modifier 2, SUMO2 ELISA Kit

Human Small Ubiquitin-related Modifier 2, SUMO2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.26ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.26ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Small Ubiquitin-Related Modifier 2

Code

E6184Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.26ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed: 26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed: 18408734, PubMed: 18538659, PubMed: 21965678, PubMed: 9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed: 24105744).

UniProt accession

MASS(Da)

10,871

GeneID

6613

Synonyms

HSMT3;Sentrin-2;Small ubiquitin-related modifier 2;SMT3 homolog 2;Smt3B;SUMO 2;SUMO2;SUMO-2;SUMO-3;Ubiquitin-like protein SMT3B

Gene names

SUMO2

Research area

Cell Biology

Target protein

SUMO2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6184Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.369 2.271 2.21
2.172
600ng/L 1.258 1.25 1.189
1.242
300ng/L 0.761 0.752 0.692
0.744
150ng/L 0.476 0.44 0.38
0.405
75ng/L 0.234 0.246 0.186
0.259
0ng/L 0.058 0.061 0
0.063

Precision

We measured random samples of E6184Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 227.6 13.93 6.1
Intra-Assay 2 18 788.3 23.34 3
Intra-Assay 3 18 453.3 27.03 6

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E6184Hu

    $320.00

  • 96T

    E6184Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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