Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Spindlin 1, SPIN1 ELISA Kit

Human Spindlin 1, SPIN1 ELISA Kit

Human Spindlin 1, SPIN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 8.05ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 8.05ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Spindlin 1

Code

E6485Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

8.05ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed: 2$458.00|$300.009551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes (PubMed: 21960006). May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption.

UniProt accession

MASS(Da)

29,601

GeneID

10927

Synonyms

Ovarian cancer-related protein;SPIN 1;SPIN1;Spindlin1;Spindlin-1

Gene names

SPIN1

Research area

Others

Target protein

SPIN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6485Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.067 2.082 2.05
2.097
800ng/L 1.315 1.424 1.392
1.533
400ng/L 0.57 0.58 0.548
0.59
200ng/L 0.437 0.416 0.384
0.395
100ng/L 0.212 0.212 0.18
0.213
0ng/L 0.021 0.032 0
0.043

Precision

We measured random samples of E6485Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 224 11.42 5.1
Intra-Assay 2 18 534.5 21.75 4.1
Intra-Assay 3 18 868.6 27.19 3.1

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E6485Hu

    $320.00

  • 96T

    E6485Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up