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Home > Product > ELISA Kit > Human Breast Cancer Anti-Estrogen Resistance Protein 3, BCAR3 ELISA Kit

Human Breast Cancer Anti-Estrogen Resistance Protein 3, BCAR3 ELISA Kit

Human Breast Cancer Anti-Estrogen Resistance Protein 3, BCAR3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.33ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.33ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Breast Cancer Anti-Estrogen Resistance Protein 3

Code

E6566Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.33ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers (PubMed: 24216110). Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis (PubMed: 24216110). Promotes insulin-mediated membrane ruffling (By similarity). In response to vasoconstrictor Peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1 (PubMed: 19086031). Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity (By similarity). May inhibit TGFB-SMAD signaling, via facilitating BCAR1 and SMAD2 and/or SMAD3 interaction (By similarity). Regulates EGF-induced DNA synthesis (PubMed: 18722344). Required for the maintenance of ocular lens morphology and structural integrity, potentially via regulation of focal adhesion complex signaling (By similarity). Acts upstream of PTPRA to regulate the localization of BCAR1 and PTPRA to focal adhesions, via regulation of SRC-mediated phosphorylation of PTPRA (By similarity). Positively regulates integrin-induced tyrosine phosphorylation of BCAR1 (By similarity). Acts as a guanine nucleotide exchange factor (GEF) for small GTPases RALA, RAP1A and RRAS (By similarity). However, in a contrasting study, lacks GEF activity towards RAP1 (PubMed: 22081014).

UniProt accession

MASS(Da)

92,566

GeneID

8412

Synonyms

BCAR 3;BCAR3;Breast cancer anti-estrogen resistance protein 3;Novel SH2-containing protein 2;SH2 domain-containing protein 3B

Gene names

BCAR3

Research area

Others

Target protein

BCAR3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6566Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.549 2.454 2.404
2.359
800ng/L 1.524 1.48 1.43
1.436
400ng/L 0.924 0.84 0.791
0.756
200ng/L 0.45 0.462 0.413
0.474
100ng/L 0.213 0.231 0.182
0.25
0ng/L 0.059 0.049 0
0.04

Precision

We measured random samples of E6566Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 640.6 19.68 3.1
Intra-Assay 2 18 329.8 20.1 6.1
Intra-Assay 3 18 396.5 21.02 5.3

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E6566Hu

    $320.00

  • 96T

    E6566Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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