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Home > Product > ELISA Kit > Human Sphingosine Kinase 1, SPHK1 ELISA Kit

Human Sphingosine Kinase 1, SPHK1 ELISA Kit

Human Sphingosine Kinase 1, SPHK1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.045ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.045ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sphingosine Kinase 1

Code

E6956Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.045ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed: 20577214, PubMed: 23602659, PubMed: 29662056, PubMed: 24929359, PubMed: 11923095). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed: 16118219). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion (PubMed: 20577214). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (PubMed: 23935096). Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed: 28049734, PubMed: 24929359). In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed: 25417698). Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.

UniProt accession

MASS(Da)

42,518

GeneID

8877

Synonyms

Acetyltransferase SPHK1;SK 1;Sphingosine kinase 1;SPHK 1;SPHK1;SPK 1

Gene names

SPHK1

Research area

Others

Target protein

SPHK1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6956Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected

Precision

We measured random samples of E6956Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.23 0.16 4.88
Intra-Assay 2 18 5.53 0.26 4.63
Intra-Assay 3 18 9.21 0.32 3.44

Protocols

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  • Size

    Code

    Price

  • 48T

    E6956Hu

    $320.00

  • 96T

    E6956Hu

    $458.00

Add to Cart

Lead time: Within two weeks

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