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Home > Product > ELISA Kit > Mouse Sorbitol Dehydrogenase, SDH ELISA Kit

Mouse Sorbitol Dehydrogenase, SDH ELISA Kit

Mouse Sorbitol Dehydrogenase, SDH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.028mU/mL

Detection range: 0.05-20mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.028mU/mL

Detection range: 0.05-20mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Sorbitol Dehydrogenase

Code

E1153Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.028mU/mL

Detection range

0.05-20mU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols (By similarity). Is active with D-sorbitol (D-glucitol) leading to the C2-oxidized product D-fructose (PubMed: 6852349). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism (By similarity). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility and protein tyrosine phosphorylation (PubMed: 18799757). Has no activity on ethanol. Cannot use NADP(+) as the electron acceptor (PubMed: 6852349).

UniProt accession

MASS(Da)

38,249

GeneID

20322

Synonyms

L-iditol 2-dehydrogenase;Polyol dehydrogenase;SDH;Sorbitol dehydrogenase;SORD;XDH;Xylitol dehydrogenase

Gene names

SORD

Research area

Others

Target protein

SORD

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1153Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12mU/mL 2.178 2.272 2.231
2.366
6mU/mL 1.363 1.356 1.314
1.348
3mU/mL 0.607 0.626 0.585
0.646
1.5mU/mL 0.409 0.372 0.33
0.335
0.75mU/mL 0.2 0.164 0.123
0.128
0mU/mL 0.033 0.042 0
0.05

Precision

We measured random samples of E1153Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5 0.2 4.1
Intra-Assay 2 18 6.4 0.22 3.4
Intra-Assay 3 18 2.2 0.11 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E1153Mo

    $320.00

  • 96T

    E1153Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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