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Home > Product > ELISA Kit > Mouse Sialidase-3, NEU3 ELISA Kit

Mouse Sialidase-3, NEU3 ELISA Kit

Mouse Sialidase-3, NEU3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.96ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 14.96ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Sialidase-3

Code

E1179Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.96ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3 (PubMed: 17708748, PubMed: 10713120, PubMed: 11696012, PubMed: 14970224). Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes (By similarity).

UniProt accession

MASS(Da)

46,846

GeneID

50877

Synonyms

Ganglioside sialidase;Membrane sialidase;N-acetyl-alpha-neuraminidase 3;NEU 3;NEU3;Sialidase-3

Gene names

NEU3

Research area

Others

Target protein

NEU3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1179Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 1.749 1.866 1.819
1.982
2400ng/L 1.073 1.082 1.035
1.091
1200ng/L 0.636 0.534 0.487
0.431
600ng/L 0.172 0.198 0.151
0.224
300ng/L 0.088 0.085 0.039
0.082
0ng/L 0.062 0.046 0
0.031

Precision

We measured random samples of E1179Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1466 72.78 5
Intra-Assay 2 18 3497.5 123.34 3.5
Intra-Assay 3 18 2068.8 73.14 3.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1179Mo

    $320.00

  • 96T

    E1179Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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