Product overview
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Background
GRAP2 (GRB2 related adaptor protein 2), a divergent member of the GRB2/Sem5/Drk family, is an adaptor protein involved in leukocyte-specific protein-tyrosine kinase signaling. GRAP2 shares similar structural organization as GRB2 and GRAP with a central SH2 domain flanked by 2 SH3 domains. However, unlike GRB2 and GRAP, the SH2 domain and the C-terminal SH3 domain of GRAP2 contains a unique 120 amino acid glutamine/proline rich sequence. In T lymphocytes, GRAP2 interacts with LAT and SLP-76 to regulate NF-AT (nuclear factor of activated T cells) and form a signaling complex with HPK1 to mediate the JNK signaling pathway. GRAP2 also interacts with M-CSFR and the activated T cell co-stimulatory receptor CD28.
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Target protein
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6955Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
12ng/ml | 2.307 | 2.37 | 2.276 |
2.433 | |||
6ng/ml | 1.397 | 1.433 | 1.339 |
1.469 | |||
3ng/ml | 0.981 | 1.013 | 0.92 |
1.045 | |||
1.5ng/ml | 0.5 | 0.488 | 0.394 |
0.475 | |||
0.75ng/ml | 0.33 | 0.313 | 0.219 |
0.295 | |||
0ng/ml | 0.08699999999999999 | 0.094 | 0 |
0.1 |
Precision
We measured random samples of E6955Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 4.5 | 0.27 | 6 |
Intra-Assay | 2 | 18 | 6 | 0.24 | 4 |
Intra-Assay | 3 | 18 | 1.6 | 0.11 | 7 |
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