Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
20000 pg/ml | 2.348 | 2.346 | 2.234 |
2.343 | |||
10000 pg/ml | 1.36 | 1.389 | 1.277 |
1.417 | |||
5000 pg/ml | 0.796 | 0.763 | 0.651 |
0.729 | |||
2500 pg/ml | 0.4 | 0.427 | 0.315 |
0.453 | |||
1250 pg/ml | 0.259 | 0.267 | 0.155 |
0.275 | |||
625 pg/ml | 0.179 | 0.18 | 0.068 |
0.18 | |||
312.5 pg/ml | 0.133 | 0.134 | 0.022 |
0.134 | |||
0 pg/ml | 0.117 | 0.112 | 0 |
0.106 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 1772 | 96.9 | 5.5 |
Intra-assay | 2 | 20 | 3691 | 124 | 3.4 |
Intra-assay | 3 | 20 | 15378 | 350 | 2.3 |
Inter-assay | 1 | 20 | 1758 | 94.6 | 5.4 |
Inter-assay | 2 | 20 | 3660 | 199 | 5.5 |
Inter-assay | 3 | 20 | 15562 | 768 | 4.9 |
Protocols
References
Customer reviews and Q&As