Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 9000 pg/ml | 2.36 | 2.277 | 2.161 |
| 2.193 | |||
| 4500 pg/ml | 1.24 | 1.303 | 1.187 |
| 1.365 | |||
| 2250 pg/ml | 0.756 | 0.772 | 0.656 |
| 0.787 | |||
| 1125 pg/ml | 0.444 | 0.439 | 0.323 |
| 0.434 | |||
| 562.5 pg/ml | 0.283 | 0.284 | 0.168 |
| 0.284 | |||
| 281.25 pg/ml | 0.19 | 0.196 | 0.08 |
| 0.201 | |||
| 140.625 pg/ml | 0.157 | 0.154 | 0.038 |
| 0.15 | |||
| 0 pg/ml | 0.111 | 0.116 | 0 |
| 0.121 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 336 | 17.4 | 5.2 |
| Intra-assay | 2 | 20 | 1682 | 67.4 | 4 |
| Intra-assay | 3 | 20 | 7010 | 312 | 4.5 |
| Inter-assay | 1 | 20 | 336 | 34.6 | 10.3 |
| Inter-assay | 2 | 20 | 1690 | 89.6 | 5.3 |
| Inter-assay | 3 | 20 | 7049 | 305 | 4.3 |
Protocols
References
Customer reviews and Q&As
