Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
4000 pg/ml | 3.007 | 3.076 | 2.973 |
3.146 | |||
2000 pg/ml | 1.625 | 1.584 | 1.481 |
1.544 | |||
1000 pg/ml | 0.779 | 0.768 | 0.665 |
0.758 | |||
500 pg/ml | 0.407 | 0.404 | 0.301 |
0.401 | |||
250 pg/ml | 0.284 | 0.273 | 0.17 |
0.263 | |||
125 pg/ml | 0.178 | 0.177 | 0.074 |
0.177 | |||
62.5 pg/ml | 0.145 | 0.139 | 0.036 |
0.134 | |||
0 pg/ml | 0.104 | 0.103 | 0 |
0.103 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 408 | 16 | 3.9 |
Intra-assay | 2 | 20 | 950 | 21.8 | 2.3 |
Intra-assay | 3 | 20 | 2261 | 67.8 | 3 |
Inter-assay | 1 | 20 | 410 | 33.6 | 8.2 |
Inter-assay | 2 | 20 | 959 | 79.5 | 8.3 |
Inter-assay | 3 | 20 | 2283 | 146 | 6.4 |
Protocols
References
Customer reviews and Q&As