Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
3000 pg/ml | 2.139 | 2.252 | 2.146 |
2.365 | |||
1500 pg/ml | 1.287 | 1.302 | 1.196 |
1.317 | |||
750 pg/ml | 0.687 | 0.718 | 0.612 |
0.749 | |||
375 pg/ml | 0.459 | 0.43 | 0.324 |
0.401 | |||
187.5 pg/ml | 0.288 | 0.271 | 0.165 |
0.254 | |||
93.75 pg/ml | 0.195 | 0.19 | 0.084 |
0.185 | |||
46.875 pg/ml | 0.138 | 0.146 | 0.04 |
0.154 | |||
0 pg/ml | 0.099 | 0.106 | 0 |
0.113 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 225 | 10.6 | 4.7 |
Intra-assay | 2 | 20 | 1023 | 29.6 | 2.9 |
Intra-assay | 3 | 20 | 2274 | 55.1 | 2.4 |
Inter-assay | 1 | 20 | 226 | 14.9 | 6.6 |
Inter-assay | 2 | 20 | 1012 | 47.2 | 4.7 |
Inter-assay | 3 | 20 | 2281 | 112 | 4.9 |
Protocols
Product publications
Customer reviews and Q&As