Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
3000 pg/ml | 2.646 | 2.591 | 2.466 |
2.537 | |||
1500 pg/ml | 1.409 | 1.368 | 1.243 |
1.327 | |||
750 pg/ml | 0.716 | 0.745 | 0.62 |
0.775 | |||
375 pg/ml | 0.411 | 0.433 | 0.308 |
0.456 | |||
187.5 pg/ml | 0.265 | 0.275 | 0.15 |
0.285 | |||
93.75 pg/ml | 0.204 | 0.2 | 0.075 |
0.196 | |||
46.875 pg/ml | 0.148 | 0.158 | 0.033 |
0.169 | |||
0 pg/ml | 0.124 | 0.125 | 0 |
0.126 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 308 | 23.6 | 7.7 |
Intra-assay | 2 | 20 | 810 | 47.7 | 5.9 |
Intra-assay | 3 | 20 | 1977 | 80.6 | 4.1 |
Inter-assay | 1 | 20 | 311 | 16 | 5.1 |
Inter-assay | 2 | 20 | 807 | 42.8 | 5.3 |
Inter-assay | 3 | 20 | 1966 | 100 | 5.1 |
Protocols
References
Customer reviews and Q&As