Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
2500 pg/ml | 2.74 | 2.84 | 2.722 |
2.94 | |||
1250 pg/ml | 1.427 | 1.448 | 1.33 |
1.469 | |||
625 pg/ml | 0.758 | 0.753 | 0.635 |
0.748 | |||
312.5 pg/ml | 0.438 | 0.433 | 0.315 |
0.428 | |||
156.25 pg/ml | 0.276 | 0.266 | 0.148 |
0.256 | |||
78.125 pg/ml | 0.176 | 0.189 | 0.071 |
0.202 | |||
39.0625 pg/ml | 0.141 | 0.145 | 0.027 |
0.149 | |||
0 pg/ml | 0.117 | 0.118 | 0 |
0.119 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 163 | 6.8 | 4.2 |
Intra-assay | 2 | 20 | 494 | 19.2 | 3.9 |
Intra-assay | 3 | 20 | 1766 | 65.9 | 3.7 |
Inter-assay | 1 | 20 | 162 | 21.3 | 13.1 |
Inter-assay | 2 | 20 | 493 | 48.4 | 9.8 |
Inter-assay | 3 | 20 | 1761 | 150 | 8.5 |
Protocols
References
Customer reviews and Q&As