Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
2500 pg/ml | 2.499 | 2.415 | 2.319 |
2.33 | |||
1250 pg/ml | 1.441 | 1.432 | 1.336 |
1.423 | |||
625 pg/ml | 0.729 | 0.773 | 0.677 |
0.817 | |||
312.5 pg/ml | 0.435 | 0.436 | 0.34 |
0.436 | |||
156.25 pg/ml | 0.255 | 0.258 | 0.162 |
0.261 | |||
78.125 pg/ml | 0.178 | 0.168 | 0.072 |
0.158 | |||
39.0625 pg/ml | 0.137 | 0.134 | 0.038 |
0.13 | |||
0 pg/ml | 0.098 | 0.096 | 0 |
0.093 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 321 | 23.9 | 7.5 |
Intra-assay | 2 | 20 | 698 | 40.8 | 5.9 |
Intra-assay | 3 | 20 | 1789 | 70.4 | 3.9 |
Inter-assay | 1 | 20 | 320 | 16.6 | 5.2 |
Inter-assay | 2 | 20 | 699 | 36.4 | 5.2 |
Inter-assay | 3 | 20 | 1784 | 91 | 5.1 |
Protocols
References
Customer reviews and Q&As