Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
2000 pg/ml | 1.524 | 1.518 | 1.405 |
1.512 | |||
1000 pg/ml | 0.934 | 0.967 | 0.854 |
1 | |||
500 pg/ml | 0.535 | 0.567 | 0.454 |
0.599 | |||
250 pg/ml | 0.349 | 0.351 | 0.238 |
0.353 | |||
125 pg/ml | 0.238 | 0.237 | 0.124 |
0.236 | |||
62.5 pg/ml | 0.166 | 0.176 | 0.063 |
0.186 | |||
31.25 pg/ml | 0.145 | 0.146 | 0.033 |
0.148 | |||
0 pg/ml | 0.111 | 0.113 | 0 |
0.115 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 236 | 12.4 | 5.3 |
Intra-assay | 2 | 20 | 525 | 16.9 | 3.2 |
Intra-assay | 3 | 20 | 1314 | 30.2 | 2.3 |
Inter-assay | 1 | 20 | 239 | 12.8 | 5.4 |
Inter-assay | 2 | 20 | 530 | 29.3 | 5.5 |
Inter-assay | 3 | 20 | 1317 | 65.1 | 4.9 |
Protocols
References
Customer reviews and Q&As